The effect of bilirubin oxidase on serum bilirubin.
نویسندگان
چکیده
منابع مشابه
Effects of thermostability of bilirubin subfractions on serum bilirubin measurement
Bilirubin is sensitive to light and oxygen. The rate of photodegradation of unconjugated bilirubin (Bu) was nearly twice that of monoconjugated bilirubin (mBc) and three fold that of diconjugated bilirubin (dBc); delta bilirubin (Bd) was the most stable against photoirradiation. In the presence of serum albumin, conjugated bilirubin (Bc: i.e., mBc and dBc) showed the highest rate of oxidation b...
متن کاملDetermination of conjugated and total bilirubin in serum of neonates, with use of bilirubin oxidase.
A new, simple, and rapid assay for conjugated bilirubin that does not require serum-matrix standards was developed by using the enzyme bilirubin oxidase (EC 1.3.3.5). This procedure can also be modified to measure total bilirubin. Measurements from these assays were compared with results obtained with the Sigma 605 (Jendrassik-Gróf method), Sigma 550/551 (Walters-Gerarde method), and Kodak Ekta...
متن کاملMeasurement of total bilirubin by use of bilirubin oxidase.
We used an enzymatic method for measuring total bilirubin in serum. Results by this method varied linearly with bilirubin concentrations to at least 300 mg/L. The day-to-day precision (CV) of the method ranged from less than 1% to about 11% at bilirubin concentrations of 183 and 12 mg/L, respectively. Commonly used anticoagulants, serum preparation materials, and selected drugs had no effect on...
متن کاملMeasurement of direct bilirubin by use of bilirubin oxidase.
We developed an enzymatic method for measuring direct-reacting bilirubin (DBIL) in serum. At pH 4.5, bilirubin oxidase (BOX) oxidizes mono-conjugated bilirubin, di-conjugated bilirubin, and most of the delta-bilirubin to biliverdin. The resulting decrease in absorbance at 460 nm is linearly related to the concentration of DBIL in serum. Mean DBIL values in the 51 patients' sera examined by the ...
متن کاملThe effect of fasting on total serum bilirubin concentrations.
Thirty-seven healthy volunteers, 19 of whom had consistently elevated total serum bilirubin (TSB) concentrations, took part in an open, randomised cross-over study to determine the effect of fasting on TSB concentrations. The study comprised of two treatments. During one treatment period volunteers ate a standard supper but fasted for 24 h thereafter. During the other treatment period volunteer...
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ژورنال
عنوان ژورنال: Kanzo
سال: 1987
ISSN: 0451-4203,1881-3593
DOI: 10.2957/kanzo.28.926